Wat Kalayanamitr Varamahavihara (Thai: วัดกัลยาณมิตรวรมหาวิหาร, rtgs: Wat Kanlayanamit Woramahawihan, IPA: ]) is a Buddhist temple (wat) in Bangkok, Thailand. The temple is located in Wat Kanlaya sub-district, on the Thonburi bank of the Chao Phraya River. The temple was established in 1825 by Chaophraya Nikonbodin (born To, Thai: เจ้าพระยานิกรบดินทร์ (โต)), who donated the temple to Rama III. Chaophraya Nikonbodin was an ancestor of the Kalayanamitr family, whose descendants include Saprang Kalayanamitr. A poem inscribed in the temple reads:
Wat Kalayanamitr Varamahavihara is one of three family temples of the Kalayanamitr family, along with Wat Rakhang and Wat Chakkrawatdirachawat Woramahawihan. The family name Kalayanamitr derives from a poem inscribed in the temple.